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KMID : 0848120060310030073
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International Journal of Oral Biology
2006 Volume.31 No. 3 p.73 ~ p.79
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Expression of DSPP mRNA During Differentiation of Human Dental Pulp-derived Cells (HDPC) and Transplantation of HDPC Using Alginate Scaffold
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Aikawa Fumiko
Nakatsuka Michiko
Kumabe Shunji
Jue Seong-Suk
Hayashi Hiroyuki
Shin Je-Won
Iwai Yasutomo
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Abstract
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Tissue stem cells are used for the regenerative medicine. In previous study we observed hard tissue formation of human dental pulp-derived cells using alginate scaffold. In this study, we explore the ability to differentiate of the 13th passage cells with glycerol 2-phosphate disodium salt hydrate (β-GP) which accelerate calcification. Reverse transcriptase Polymerase Chain Reaction (RT-PCR), transplants using alginate scaffold and histological examination were performed. We observed the expression of DSPP mRNA on day 10 cultured cells with β-GP. In conclusion, the 13th passage cells still have an ability to differentiate into odontoblast-like cells and alginate supports the differentiation of cultured cells in the transplants.
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KEYWORD
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Odontoblast-like cell, Human, Dentinsialophosphoprotein Cell culture, Transplantation
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